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Ghost & MoreTomato-induced B cell hyperactivity is associated with secondary hyperparathyroidism and up-regulation of CD4 + CD45RA + regulatory T cells in rats.
Bone resorption caused by increased osteoclastic activity induces secondary hyperparathyroidism (SHP). SHP in turn, leads to increased levels of serum calcium and phosphate in humans. The mechanism by which SHP develops is largely unknown. In this study we investigated whether SHP induces a state of immune suppression leading to elevated levels of anti-parathyroid antibodies (aP) or increased incidence of tumors. Because of the important influence of dietary phosphate on the serum parathyroid hormone levels (PTH), we also tested whether a phosphate-rich diet could induce aP. We fed female Sprague-Dawley rats for 8 weeks with diets containing different concentrations of phosphate. The serum concentration of aP and PTH was assayed at the end of the experiment. B cells were analyzed for the expression of the activation/memory markers CD21 and CD71. Proportions of CD4 + CD45RA + cells and regulatory T cells (T reg) were determined by flow cytometry. We measured the serum calcium concentration and reabsorption of dietary phosphate. We found that SHP-induced by phosphate feeding was associated with aP with a median serum concentration of 12.9 U/mL (range, 9.8-23.0 U/mL). We detected neither an increase in the total number of B cells, nor an increase in the frequency of CD21-expressing (activated) B cells, nor in the percentage of T reg cells compared with that of control animals. However, the percentage of CD4 + CD45RA + cells was markedly increased in SHP animals. Supplementation of a phosphate-rich diet induced hyperparathyroidism but did not lead to aP, indicating that SHP is not directly responsible for inducing aP in rats.Warner Bros. continues its penchant for odd casting choices by casting the role of the devil in its upcoming Joker movie, a role that was once cast and played by Ted Levine.

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