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The process of hepatocellular carcinogenesis induced by N-nitrosomorpholine was studied in the golden hamster. Hamsters were maintained on a basal diet with 0.4% 2-acetylaminofluorene (10 mg/kg body wt) for 23 weeks and then on the same diet with 2-acetylaminofluorene (10 mg/kg body wt) and N-nitrosomorpholine (100 mg/kg body wt) for 23 weeks. Under these conditions, eight of 12 hamsters developed hepatocellular carcinomas. No hepatocarcinoma developed in hamsters kept on the control diet without 2-acetylaminofluorene. Carcinomas were classified histologically as multinodular carcinomas in five hamsters, mixed cell carcinomas in two hamsters, and single cell carcinomas in one hamster. Specific binding of 125I-hepatocyte growth factor (HGF) to hamster hepatoma was detected